U.S. Patent No. 9,474,272
Issued October 25, 2016
US Serial No. 13/838,725
Application Filed: 3/15/2013
Entitled: “Micropropagation of Alexandrian Laurel (Danae Racemosa)”
Dr. Guochen Yang and Zhongge Lu
N.C. A&T, Greensboro, NC

Abstract: Alexalexandrian-laurelandrian laurel (Danae racemosa L.) is a highly demanded evergreen shrub. However, seed germination often takes up to 12 to 18 months with a germination rate of only about 20%. Also, due to its slow-growth rate, it typically takes up to six years to produce a saleable one-gallon size plant. Germinating seeds in the presence of cytokinins and/or auxins increased in vitro shoot multiplication and seedling quality enhancement. Benzyladenine was found to balance seedling development by simultaneously accelerating shoot growth and slowing down root growth, whereas thidiazuron significantly promoted shoot multiplication and proliferation by producing 5-30 shoots per seed.